| 摘要 |
PURPOSE:To make it possible to classify a part of osteoporosis as one of autoimmune diseases by detecting an autoantibody in the blood serum of an osteoporosis patient using an osteospecific sialoprotein. CONSTITUTION:An antibody containing an osteospecific sialoprotein (BSP) as an antigen is present in the blood serum of an osteoporosis patient. A diagnostic agent therefor is present in the osseous tissue of an osteoporosis patient and contains an antigen which reacts on the antibody in the blood serum of human osteoporosis patient but does not react on the antibody in the blood serum of healthy human. For example, BSP extracted from the bone of a mammal, e.g. a bovine, is purified by ion exchange chromatography to produce a diagnostic agent. The purifies antigens are then fixed to a plastic microplate having 96 holes and caused to react on a sample, i.e., a blood serum of human, and an enzyme-labeled secondary antibody is further caused to react before the color development of enzyme is detected.
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