发明名称 |
METHOD FOR PURIFICATION OF A MIXTURE OF HYDROXAMATE DERIVATIZED PROTEIN AND NATIVE PROTEIN |
摘要 |
The invention relates to a method for purification of a mixture of hydroxamate derivatized protein/proteins and native protein which is characterized by treating the mixture with immobilized metal and thereby adsorbing the hydroxamate derivatized protein/proteins on the immobilized-metal and recovering the native protein. The protein could be IGF-I. It also relates to a process for the production of a native protein which is characterized by expression of the protein as a fusion protein, cleavage of the fusion protein by hydroxylamine, separation of native protein from hydroxamate derivatized protein by adsorbing the hydroxamate derivatized protein on immobilized metal and directly recovering the native protein. The use of immobilized metal affinity chromatography for separation of native protein from hydroxamate derivatized protein is also claimed.
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申请公布号 |
WO9514035(A1) |
申请公布日期 |
1995.05.26 |
申请号 |
WO1994SE01088 |
申请日期 |
1994.11.17 |
申请人 |
PHARMACIA AB;WIKSTROEM, PER |
发明人 |
WIKSTROEM, PER |
分类号 |
C07K1/107;C07K1/14;C07K1/22;C07K14/65;(IPC1-7):C07K14/00;C07K1/00 |
主分类号 |
C07K1/107 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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