Spezifische Bindungssubstanzen für Antikörper und deren Verwendung für Immunoassays oder Vakzine

摘要

The invention pertains to an immunological binding substance for antibodies and its use in immunoassays and as vaccine. The immunological binding substance, which specifically binds with an antibody specifically directed to an epitope with a natural amino acid sequence and which either bears a detectable marking or is solid-phase bonded or can be bonded to a solid phase via a binding molecule or is bonded to an immunogenic molecule containing a T-cell epitope or to a carrier protein molecule, is characterized in that the specific binding substance constitutes an amino acid sequence corresponding to the natural epitope, wherein the sequence at least to one peptide bond or to one amino acid is changed in that a) one or more -CO-NH peptide binding groups are replaced by a two- or three-atom bridge different from them, b) amino acid side chains are shortened by a -CH2 group or lengthened by a -CH2, -S, -O, -NH, -SO2 or -CO group, c) one or more amino acids are replaced by a nonbiogenic L or D amino acid of the HR'N-Y-COOH type, where Y is a (CH2)n group with n = 2-8 or a -CH-(CH2)m- CR<1>R<2>R<3> group with m = 0-3, where R<1>, R<2> and R<3>, which may be the same or different, are hydrogen, a branched or unbranched C1-C4 alkyl radical or a phenyl, naphthyl or O-. S- or N-containing, 5- or 6-membered heteroaryl radical, which can be methyl, halide, NH2, OH or carboxy substituted, or where Y is a CHR group, where R is a side chain of a natural amino acid in which a -CH2 group is replaced by -S, NH, -CH=, -SO2, -CO, or -O, or in which one or more H atoms are methyl, NH2, carboxyl, SH, halide or hydroxy substituted, where in all cases Y forms a grouping unlike that of any natural amino acid or where R' is methyl, ethyl or phenyl and Y is a CHR group in which R is a side chain of a natural amino acid..