| 摘要 |
PURPOSE:To obtain the enzyme useful as an enzyme for measuring 3-butyric acid by culturing a bacterium belonging to the genus Alcaligenes, capable of producing 3-hydroxybutyric dehydrogenase. CONSTITUTION:A bacterium belonging to the genus Alcaligenes, capable of producing 3-hydroxybutyric dehydrogenase, preferably Alcaligenes sp. No.981 (FERM BP-2,570), is cultured in a medium and 3-hydroxybutyric dehydrogenase is collected from its culture product. The bacterium is cultured under aerobic conditions usually at 15-37 deg.C. |
