PREPARATION PROCESS OF RICKETTSIA TSUTSUGAMUSHI ANTIGEN

摘要

The preparation method for Rickettsia tsutsugamushi (I) antigen (II) consists of (1) injecting (I) into the vitelline membrane (III) of specific pathogen free chicken and culturing at 35 deg. C for 9-11 days, (2) transferring (I) for 50 generations (almost 2 years) as step 1, (3) homogenizing (III), (4) inactivating (I) by adding 0.05-0.5% of 37% formalin solution at 4-37 deg. C for 5-60 days, (5) passing through the column packed with Amberlite to discard yolk components, (6) centrifuging in Percoll gradient and recovering (II) at 1.06-1.07 density. (I) is used as Gilliam type ATCC VR 312 and/or Kamp type ATCC VR 150.