发明名称 |
Verfahren zur Reinigung Vitamin K-abhängiger Gerinnungsfaktoren durch Chromatographie |
摘要 |
A process for the purification of vitamin K dependent coagulation factors by affinity chromatography wherein fractions of a source containing said vitamin K dependent factors are a) subjected to chromatography on a material modified with 2-hydroxyaminopropyl groups bonded to spacers having more than 3 carbon atoms, said source being applied from a solution whose ionic strength is chosen such that said vitamin K dependent coagulation factors are adsorbed on the above anion-exchange material, washing is optionally performed with an appropriate buffer solution, then, after separation of the eluate, b) a buffer solution of higher ionic strength than that used in step a) is contacted with the affinity chromatographic material such that said vitamin K dependent factors are eluted, then this fraction is either processed further to give a PPSB preparation, or these fractions containing the factors are pooled and then said fractions c) are subjected to a second chromatographic step in the form of a heparin affinity chromatography.
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申请公布号 |
DE19504852(A1) |
申请公布日期 |
1996.08.22 |
申请号 |
DE19951004852 |
申请日期 |
1995.02.15 |
申请人 |
OCTAPHARMA AG, ZIEGELBRUECKE, CH |
发明人 |
JOSIC, DJURO, WIEN, AT;MORFELD, FRANK, 67560 ESCHBORN, DE |
分类号 |
C12N9/64;(IPC1-7):C07K14/745;C07K1/22 |
主分类号 |
C12N9/64 |
代理机构 |
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主权项 |
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地址 |
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