SEQUENCE MODIFICATION OF OLIGONUCLEOTIDE PRIMERS TO MANIPULATE NON-TEMPLATED NUCLEOTIDE ADDITION
摘要
The present invention is directed to methods for resisting or promoting template independent nucleotide addition to the 3' terminus of a DNA duplex. The process comprises amplifying a target nucleic acid using primers which comprise a 5' terminal sequence which resists or promotes non-templated nucleotide addition to the 3' terminus of the complementary nucleic acid strand. The invention is also directed to a kit for cloning 3' nucleotidylated duplex DNA.
申请公布号
WO9716566(A1)
申请公布日期
1997.05.09
申请号
WO1996US16544
申请日期
1996.10.15
申请人
THE GOVERNMENT OF THE UNITED STATES OF AMERICA, RE;SMITH, JEFFREY, R.;CARPTEN, JOHN, D.;BROWNSTEIN, MICHAEL, J.
发明人
SMITH, JEFFREY, R.;CARPTEN, JOHN, D.;BROWNSTEIN, MICHAEL, J.
