Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR

摘要

The invention relates to an enzyme mixture consisting of two thermostable DNA polymerases with and without proof-reading activity, a thermostable pyrophosphatase and other auxiliaries for PCR and its use for the multiplication of particularly long single and double-stranded nucleotide fragments. The process for multiplying the long DNA fragments is distinguished in particular by the use of the enzyme mixture, a tricine-NH3 buffer and an elongation temperature of about 68 DEG C.