A METHOD OF FLOW CYTOMETRIC DETERMINATION OF TELOMERASE ACTIVITY

摘要

There are provided a quantitative and simple method of determining telomerase activity and a method of detecting a telomerase positive cell, said methods comprising: (i) a step of conducting elongation reaction of telomerase substrate with telomerase in cells; (ii) a step of subjecting the resultant elongation reaction product to base binding chain reaction in cells; and (iii) a step of quantitatively determining fluorescent light derived from a reaction product in cells using flow cytometry.