| 摘要 |
Use of at least one complex form of calmodulin-like skin protein (CLSP) (A) derived at least partly from a polypeptide of amino acid sequence encoded by a nucleic acid sequence of SEQ ID NO: 1 (comprising a fully defined 858 base pairs as given in the specification) or its analog or fragment, as in vitro or ex vivo characterization tool of a chronological aging of an epithelium, is claimed. Independent claims are included for: (1) process-I for non-invasive characterizing an epithelium condition, comprising: (a) determining a content of at least one complex form in a sample of the epithelium surface; and (b) comparing the content determined in step (a) at a reference value; (2) process-II for screening of anti-aging active ingredients, comprising: (a1) contacting, at least one cell type containing the complex form with at least one anti-aging active ingredient to be tested, under conditions favorable to the expression of the complex form; (b1) determining a content of the complex form; and (c1) comparing the content determined in step (b1) at a content of the complex form determined in the absence of chemical or biological compound to be tested; (3) cosmetic process to characterize the effectiveness of a cosmetic or therapeutic treatment to prevent and/or treat the signs of skin aging linked to chronological aging in an individual, comprising at least qualitative and quantitative characterization of the complex form; and (4) non-therapeutic process, for characterizing the effectiveness of a treatment capable of preventing and/or treating the signs of skin aging in an individual, comprising: (i) placing at least a first sample of skin surface representative of the person; (ii) quantifying the level of the sample particularly using enzyme-linked-immunosorbent assay technique, at least as complex form; (iii) repeating the steps (i) and (ii) on a second sample of skin surface representative of the individual; and (iv) comparing the results obtained from steps (ii) and (iii), preferably to derive information related to at least one effect of the treatment, where the first and second samples of skin surface correspond to different treatment steps. ACTIVITY : Dermatological. MECHANISM OF ACTION : None given. |
