Method of identifying a biologically-active composition from a biofilm

摘要

The subject invention provides materials and methods that effectively support innate immunity and/or disperse pathogenic biofilms using readily available, nontoxic, natural substances, while supporting restoration of normal microbiotic homeostasis. In one embodiment, the subject invention provides anti-biofilm compositions comprising one or more probiotic organisms, anti-microbial honey, and other ingredients such as prebiotic compounds, other hive products, green tea derivatives, other plant derivatives, and vitamin D3.

主权项

1. A method for identifying and selecting a biologically-active composition, wherein said method comprises: (1) growing a microbial biofilm on a substrate wherein said substrate upon which said biofilm is grown is contained within a reactor vessel and is contacted with a biological growth medium within said reactor vessel; (2) harvesting said biofilm by removing the biofilm from the substrate and separating the biofilm from the growth medium thereby obtaining a fraction comprising biofilm cellular mass; (3) testing said fraction of biofilm cellular mass for biological activity; (4) based on results of the testing in step (3), determining whether said fraction has biological activity; and (5) selecting, as the biologically-active composition, the fraction that is found to have biological activity; wherein said biological activity is anti-microbial activity or anti-inflammatory activity.