| 摘要 |
<p>The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell, where the gene is expressible, isolating the protein from the cell without reducing it and subjecting the isolated protein to a folding treatment. Preferably, the immunoglobulin superfamily protein is selected from the group consisting of Class I and Class II histocompatibility molecules and beta 2 microglobulin (beta2m). Other embodiments of the invention is a stable peptide free MHC protein obtainable by a process of the invention and a kit comprising a MHC class I heavy chain and a abeta2m allowing the recipient to produce and measure or detect a functional MHC class I protein to which a peptide, which is capabe of binding to said MHC class I protein, can be added leading to the generation of a functional MHC class I protein.</p> |
