发明名称 |
SYSTEME MICROFLUIDIQUE ET PROCEDE POUR ISOLER ET QUANTIFIER AU MOINS UNE SOUS-POPULATION DE CELLULES A PARTIR D'UNE POPULATION DE CELLULES. |
摘要 |
Disclosed are a microfluidic system (1) configured to receive cell populations and further configured to simultaneously isolate and quantify at least one sub-population of cells for each cell population, and related methods of using the system. The system comprises a substrate having networks of microchannels comprising a first sorting unit configured to isolate, by magnetic attraction, cells of interest in the population in at least one first sorting microchannel. The network comprises a second unit for simultaneous sorting and counting comprising at least one second sorting microchannel defined by a closed wall having an inner face provided with at least one functionalised electrode configured to trap a sub-population. The second unit further comprising means for counting the sub-population by impedance spectroscopy. The second sorting microchannel can have at least one pair of opposing functionalised electrodes and at least one pair of second micro-coils for trapping the cells of interest arranged in the wall facing the electrodes and controlling successive attraction/release cycles that alternate between the micro-coils. |
申请公布号 |
FR3009082(B1) |
申请公布日期 |
2016.10.21 |
申请号 |
FR20130057410 |
申请日期 |
2013.07.26 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE |
发明人 |
GUE ANNE-MARIE;REYBIER KARINE;SUDOR JAN;CARGOU SEBASTIEN;MONTROSE ARMELLE |
分类号 |
G01N15/10;C12Q1/06;G01N27/02;G01N27/26;G01N33/543;G01N33/80 |
主分类号 |
G01N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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