| 发明名称 | Method for producing protein | ||
| 摘要 | The present invention relates to a recombinant host cell, wherein the cell is modified to increase the expression levels of Ero1 and XBP1 relative to the expression levels of Ero1 and XBP1 in an unmodified cell. The present invention also relates to a method of producing a recombinant protein of interest comprising expressing the recombinant protein of interest in the recombinant host cell. | ||
| 申请公布号 | US9476081(B2) | 申请公布日期 | 2016.10.25 |
| 申请号 | US201414451975 | 申请日期 | 2014.08.05 |
| 申请人 | UCB Biopharma SPRL | 发明人 | Cain Katharine Lacy;Peters Shirley Jane;Stephens Paul Edward |
| 分类号 | C12N15/63;C12P21/00;C07K14/47;C07K16/00;C07K16/18;C07K16/40;C12N9/02;C12P21/02 | 主分类号 | C12N15/63 |
| 代理机构 | McDonnell Boehnen Hulbert & Berghoff LLP | 代理人 | McDonnell Boehnen Hulbert & Berghoff LLP |
| 主权项 | 1. An isolated recombinant polynucleotide, comprising: (a) a recombinant polynucleotide sequence encoding Endoplasmic Reticulum Oxidoreductin 1 (Ero1) or a variant thereof which retains at least 80% of the activity of an endogenous Ero1; and (b) a recombinant polynucleotide sequence encoding X-box binding protein 1 (XBP1) or a variant thereof which retains at least 80% of the activity of the endogenous XBP1. | ||
| 地址 | Brussels BE | ||