| 摘要 |
<p>The present invention provides a method for detecting the amplification of a target polynucleotide. In the method, the target polynucleotide has annealed thereto an oligonucleotide probe which incorporates a modified nucleotide having a fluorescent characteristic which is modified by one or more neighbouring unmodified nucleotides. An oligonucleotide primer annealed to the target polynucleotide is extended with a polymerase having a 5'-3' exonuclease activity, and the change in fluorescence is detected as the oligonucleotide probe is degraded by the exonuclease activity of the poloymerase as the polymerase extends the primer and modification of the fluorescent characteristic of the modified nucleotide is reduced.</p> |
