发明名称 METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES
摘要 The present invention is directed to methods for the production and isolatio n of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), whi ch may be single- or double-stranded. Additionally, the invention concerns selection and isolatio n of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.
申请公布号 CA2287525(A1) 申请公布日期 2001.05.12
申请号 CA19992287525 申请日期 1999.11.12
申请人 LIFE TECHNOLOGIES, INC. 发明人 JESSEE, JOEL A.;GRUBER, CHRISTIAN E.
分类号 C12N15/09;C07H21/00;C07H21/04;C12N15/10;C12P19/34;C12Q1/68;(IPC1-7):C12N15/10;C12N15/66 主分类号 C12N15/09
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