发明名称 |
Sex-chromosome-specific proteins, species specific and sperm specific proteins and methods for their identification and isolation |
摘要 |
Sex-chromosome-specific molecules associated with animal sperm cell membranes, and the method for isolating them, are described. An X chromosome specific molecule, a protein, having an apparent molecular weight of 32 kDa on SDS-PAGE is specifically described. The method involves preparing a cell membrane fraction from animal sperm cells; treating the cell membrane fraction with one or more substances which bind to X or Y chromosome specific molecules in the cell membrane fraction to form conjugates between the X or Y chromosome specific molecules and the substances; separating the material in the cell membrane fraction which does not bind to the substances to obtain a subfraction containing sex-chromosome-specific molecules. Methods of using the sex-chromosome-specific molecules for sperm sexing are also described.
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申请公布号 |
US2001041348(A1) |
申请公布日期 |
2001.11.15 |
申请号 |
US20000731281 |
申请日期 |
2000.12.07 |
申请人 |
BLECHER STAN;DETMAR JACQUELINE |
发明人 |
BLECHER STAN;DETMAR JACQUELINE |
分类号 |
A01K67/00;A01K67/027;C07K14/47;C07K14/705;C07K16/18;C12N15/09;(IPC1-7):G01N33/537;G01N33/543;C07H21/04 |
主分类号 |
A01K67/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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