Image-based quantitation of molecular translocation

摘要

The use of an imaging system, cell compartment markers, and molecular markers in methods for correlating the movement of molecules within a cell to a particular compartment are provided, including measuring and correlating molecule movement in adherent and non-adherent cells.

主权项

1. A method for measuring molecular movement in a cell, comprising:
contacting the cell with at least one marker; imaging the cell with a detector; creating a mask; and measuring the molecular movement in the cell based at least in part on a correlation between the mask and the at least one marker.