MEDIUM AND METHOD FOR DETECTING SUGAR FERMENTATION OF MICROORGANISMS IN SHORT TIME

摘要

PURPOSE: A medium and method for inspecting the sugar fermentation of microorganisms in a short time are provided, thereby rapidly detecting the sugar fermentatability of microorganism. CONSTITUTION: The medium for detecting the sugar fermentation of a microorganism in a short time is made by adding 10g of glucose, 2g of peptone, 0.3g of dipotassium phosphate, 0.03 to 0.05g of bromthymol blue(BTB) into 1 liter of distilled water; adjusting the hydrogen concentration to pH 7.1; and further adding 1 to 2g of yeast extract and 3 to 4g of beef extract into the medium. The method comprises the steps of: inserting the medium into each tube; inoculating a microorganism into the medium; coating mineral oil on the medium and cultivating it at 37 deg.C for 4 hours; and analyzing the color of the medium to determine the microorganism's sugar fermentatability.