发明名称 |
Recombinant expression vector of human parathyroid hormone |
摘要 |
The present invention relates to a recombinant expression vector which is prepared by inserting a human parathyroid hormone gene containing a urokinase-specific cleavage site into an L-arabinose inducible vector containing a phosphoribulokinase gene fragment of Rhodabacter sphaeroides or its mutated gene as a fusion partner, or its mutate gene as a fusion partner, a recombinant microorganism transformed with the said expression vector, and a process for preparing human parathyroid hormone on a large scale by cultivating the said microorganism in a medium containing L-arabinose. In accordance with the invention, a recombinant human PTH having the same activity of the native human PTH can be prepared in a high yield through the precise control of induction by a manufacturing process which comprises a step of inducing expression of fusion protein in the microorganism transformed with the recombinant expression vector by L-arabinose.
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申请公布号 |
US6500647(B1) |
申请公布日期 |
2002.12.31 |
申请号 |
US20000463282 |
申请日期 |
2000.01.20 |
申请人 |
MOGAM BIOTECHNOLOGY RESEARCH INSTITUTE |
发明人 |
JUNG EUN-KYUNG;PARK DOO-HONG;CHUNG SOO-IL |
分类号 |
C12N15/09;C07H21/04;C07K17/00;C08H1/00;C12N1/21;C12N15/16;C12P21/00;C12P21/02;C12R1/19;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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