СПОСОБ ВЫЯВЛЕНИЯ ВИРУСА ИРТ КРС В ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ С ПОСЛЕДУЮЩЕЙ ДИФФЕРЕНЦИАЦИЕЙ ВАКЦИННОГО ШТАММА ТК-А ОТ ЭПИЗООТИЧЕСКИХ ШТАММОВ И ИЗОЛЯТОВ ПРИ ПОМОЩИ ПДРФ-АНАЛИЗА

摘要

FIELD: biotechnology, genetic engineering, virology. ^ SUBSTANCE: invention proposes a method for isolating virus IRT KRS in polymerase chain reaction. Method involves carrying out polymerase chain reaction with primers B1 and B2 followed by differentiation of detected DNA in case of positive result in PCR reaction. Result is considered to be positive if PCR product corresponds to size of fragment consisting of 464 pair bases. For differentiation PCR product is treated with (restriction) endonuclease Sac II. As result, the presence of one fragment with length 464 pair bases corresponds to the vaccine strain TK-A and two fragments of size 343 and 121 pair bases correspond to epizootic strains and isolates. ^ EFFECT: improved detecting method and analysis. ^ 1 dwg, 3 tbl, 6 ex