MASSIVE PARALLEL PRIMER DIMER-MEDIATED MULTIPLEXED SINGLE CELL-BASED AMPLIFICATION FOR CONCURRENT EVALUATION OF MULTIPLE TARGET SEQUENCES IN COMPLEX CELL MIXTURES

摘要

The present invention provides methods and compositions for parallel single cell-based multiplexed primer dimer-mediated amplification of multiple target sequences in complex cell mixtures. The invention provides primer dimer-mediated concatenation of multiple targets in multi-target fusion amplicon products for evaluation of two or more target sequences as present individual cells across a population or mixture of cells.