| 摘要 |
PURPOSE:To prepare a polysaccharide in high yield, by cultivating a new strain, Pseudomonas sp. GD-96, belonging to the genus Pseudomonas, in a bacterium cultivating medium comprising methanol as a carbon source. CONSTITUTION:Methanol as a main carbon source is added to a medium comprising a nitrogen source, e.g., NH4NO3, KH2PO4, MgSO4, CaCl2, vitamin, etc. The strain Pseudomonas sp. GD-96(FERM-P 5194) is inoculated on a medium to which methanol is added, and cultivated usually under aerobic conditions. For example, the cultivation is carried out by cultivating under agitation or agitation with aeration in a pH of the medium 6.5-7.5. After cultivation, the culure solution is diluted by 10-20 times, the strain and solid substance are removed by filtration or centrifugation, double amount of acetone is added to give a white fibrous crude polysaccharide, which is purified and dried under freezing to give a white spongy purified polysaccharide. |
