Method of producing antiserum for histological identification of endocrine-active cells, especially cells producing substance P

摘要

Method of producing an antiserum to a peptide or protein component from endocrine-active cells for identification of such cells by applying the antiserum to a microscopic section. Tissue with said endocrinally active cells is disintegrated, and from the disintegrated product one separates in familiar fashion by centrifuging and filtering steps an insoluble granule fraction, which contains said peptide or protein component. The insoluble granule fraction is suspended in aqueous solution and the antiserum is prepared by using the aqueous suspension. Especially intended are antisera to a granule fraction from tumours, especially carcinoids producing substance P.