Substrates and methods for collection, stabilization and elution of biomolecules

摘要

A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.

主权项

1. A method for extracting, stabilizing and eluting a protein or peptide from a biological sample disposed on a non-dissolvable dry solid substrate, wherein the non-dissolvable dry solid substrate comprises cellulose, cellulose acetate or glass fiber, or a combination thereof, and wherein the non-dissolvable dry solid substrate is a melezitose-impregnated solid substrate that is prepared by dipping the substrate in a melezitose solution having a melezitose concentration in a range of 10% to 30% on a weight per volume basis, the method comprising:
contacting the biological sample to the melezitose-impregnated solid substrate without solubilizing the melezitose-impregnated solid substrate; drying the biological sample to a water content of less than 2%; and eluting the a protein or peptide from the biological sample dried on the melezitose-impregnated solid substrate by rehydrating the melezitose-impregnated solid substrate in an elution buffer.