Method of measuring hydrogen peroxide or of measuring an enzyme or biochemical substrate liberating the peroxide.

摘要

To an aqueous sample containing hydrogen peroxide there are added (a) an aromatic hydroxy compound (e.g. a phenol or naphthol which may have substituents), (b) a diamine compound (e.g. an aniline compound, e.g. a p-amino aniline), and (c) an oxidase enzyme (peroxidase or catalase), and after mixing and standing for 5 minutes to form a quinomic dye the depth of color of the dye in solution is measured and compared to a control, to measure the amount of the H2O2. An enzyme (e.g. uricase) and its substrate substance (e.g. uric acid) from which H2O2 is liberated can be mixed to form the H2O2 sample and from the dye measurement the amount of either the enzyme or its substrate can be calculated. The method is reproducible and rapid and can be used in an automatic analyzer.