| 摘要 |
<p>Immunosuppressive factor of the following properties: (1) molecular weight: 45,000 to 65,000 and 150,000 to 200,000 according to gel filtration and 31,000 according to SDS-polyacrylamide gel electrophoresis; (2) isoelectric point: 4.6 to 4.8; (3) being elutable at a salt concentration of 0.31 to 0.32 M by FPLC-mono Q anion exchange chromatography; (4) not adsorbable to immobilized concanavalin A sepharose and Blue sepharose; (5) non-sensitive to deoxyribonuclease, ribonuclease, papain, and periodic acid, and sensitive to trypsin, α-chymotrypsin, and pronase; (6) stable at pH 2 to 10; (7) stable for a long time at 4°C and partly deactivated by heat treatment at 56°C or 90°C for 30 minutes; (8) not inhibitable by 2-mercaptoethanol, levamisole, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, α-methyl-D-mannose, L-arginine or L-ornithine; (9) inhibiting production of antibody; (10) inhibiting blastogenic transformation of lymphocytes; (11) inhibiting cell division and cell multiplication; and (12) having specific structural amino acids and specific contents thereof. This immunosuppressive factor has an excellent immunosuppressing effect and is useful as medicine or reagent.</p> |
