Serum-free animal cell culture medium and methods for the primary culture and production of cell lines using this medium

摘要

PCT No. PCT/FR84/00080 Sec. 371 Date Nov. 21, 1984 Sec. 102(e) Date Nov. 21, 1984 PCT Filed Mar. 23, 1984 PCT Pub. No. WO84/03710 PCT Pub. Date Sep. 27, 1984.A serum-free animal tissue culture medium contains a mixture of six fatty acids and albumin or dextran. The medium is particularly adapted for the primary culture of rat liver epithelial cells and possibly in the presence of hormones and/or growth factors, for obtaining cell lines, in particular of myelomae and hybridomae.