Immunoassay for measuring the concentration of antigen in a sample

摘要

An immunoassay comprising immobilizing antibody in a matrix for electrophoresis; immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis; either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; and measuring the concentration of antigen in the sample, characterized by using as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance, moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis, reacting the substrate with the label enzyme to convert the same into a fluorescent substance, and measuring the concentration of the fluorescent substance in the electrolyte solution.