| 摘要 |
The method for preparing a microbial insecticide by Bacillus thuringiensis isolated in soils is presented. Thus, B.thuringiensis is seed-cultured in TPB (Triptose Phosphate Broth) at 30oC for 12hrs. The main culture is carried out in the production medium containing 25g/l of glucose, 15g/l of corn starch, and trace elements consisted of 1g/l of KH2PO4, 1g/l of K2HPO4, 0.3g/l of MgSO4.7H2o, 1.0g/l of CaCO3 0.02g/l of CuSO4.5H2O, 0.04g/l of MnSO4, 0.01g/l of FeSO4.7H2O and 0.02g/l of ZnSO4.7H2O. The culture condition is as follows: Temp. is 28-32oC and initial Ph being 6.8 - 7.2. The initial aeration is 0.5 - 1.0v.v.m.
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