发明名称 |
Idiotype and anti-idiotype antibodies useful in virus detection |
摘要 |
The invention describes a new process for the production of anti-viral diagnostics, as well as an example of one antibody useful in detecting a wide variety of pathogenically important alphaviruses. The process includes, but is not limited to, the isolation of monoclonal antibodies directed against internal viral components. Such antibodies may be generated by conventional immunization in vivo or in vitro using subviral particles or synthetic peptides corresponding to specific viral proteins. Alternatively, synthetic peptides derived from the cytoplasmic domains of envelope proteins may be used to elicit monoclonal idiotypic and anti-idiotypic antibodies following immunization in vitro and/or in vivo. The anti-alphavirus antibody included in the invention is an example of the type of reagent produced. It was produced as an anti-idiotype against monoclonal antibodies to a synthetic peptide derived from Semliki Forest virus. The monoclonal anti-idiotype is specific for the genome-containing nucleocapsid structure not only of Semliki Forest virus, but of every alphavirus tested thus far.
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申请公布号 |
US4910131(A) |
申请公布日期 |
1990.03.20 |
申请号 |
US19870137237 |
申请日期 |
1987.12.23 |
申请人 |
MELLMAN, IRA S.;VAUX, DAVID;HELENIUS, ARI H. |
发明人 |
MELLMAN, IRA S.;VAUX, DAVID;HELENIUS, ARI H. |
分类号 |
G01N33/569;G01N33/68 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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