摘要 |
Novel hybrid thermostable (1,3-1,4)- beta -glucanases, their use in food manufacturing and feed manufacturing, DNA fragments encoding such glucanases, organisms expressing the DNA fragments and a method for producing the thermostable (1,3-1,4)- beta -glucanases. Hybrid fusion genes encoding Bacillus (1,3-1,4)- beta -glucanases were constructed, the gene products of which are more thermostable than any (1,3-1,4)- beta -glucanase known until now. The hybrid genes were constructed by reciprocal exchanges of the amino-terminal and carboxy-terminal parts of the beta -glucanase encoding genes from Bacillus amyloliquefaciens and Bacillus macerans. The resulting thermostable (1,3-1,4)- beta -glucanases retain a significant enzymatic activity at temperatures exceeding 65 DEG C and at pH values below 5.0. |
申请人 |
CARLSBERG A/S;AKADEMIE DER WISSENSCHAFTEN DER DDR |
发明人 |
BORRISS, RAINER;HOFEMEISTER, JURGEN;THOMSEN, KARL K.;OLSEN, OLE;VON WETTSTEIN, DIETRICH |