Extraction composition, test kit and their use to extract or determine herpes simplex viral antigen.

摘要

An extraction composition has been found useful for extracting antigen from Herpes simplex virus. This composition has a pH of from 8.5 to 12, and comprises an alcoholamine or salt thereof, a nonionic surfactant comprised of a condensation product of an alkylphenol and ethylene oxide, cholic acid or a salt or derivative thereof and an anionic surfactant. Extraction of antigen is accomplished by contacting the extraction composition with a specimen suspected of containing herpes organisms under suitable conditions. Extracted antigen can be determined by forming an immunological complex with antibodies thereto and by detecting that complex. The extraction composition can be supplied as part of a diagnostic test kit.