Process for 5'-phosphorylation of nucleic acids

摘要

Compounds of the formula <IMAGE> are phosphorylating reagents which react with acylatable hydrogen atoms with elimination of the amine HNR3R4. The resulting compounds are oxidized to give the corresponding phosphate, thiophosphate or selenophosphate derivatives, and the radicals R1 and R2 are then split off by means of bases. The chemical nature of the radicals R1, R2, R3 and R4 accordingly depends on the requirements for these cleavage reactions.