STABLE PROPAGATION OF MODIFIED FULL LENGTH CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR PROTEIN CDNA IN HETEROLOGOUS SYSTEMS

摘要

A modified DNA sequence encoding full length cystic fibrosis transmembrane conductance regulator protein is provided to facilitate propagation and/or expression of the protein in living cells a nd in particular, bacterial cells. The modified DNA se- quence comprises at least one of the 13 base pair repeat of exon 6b of the normal gene encoding the conductance regulator pro- tein, as one or more normal nucleotides of the 13 base pair repeat substitut ed with an alternate nucleotide which however conti- nues to code for the corresponding normal amino acid. Mammalian cells transfected with a vector containing the modified DNA sequence enhances chlorine conductance through the cell wall.