发明名称 |
Modified avidin-biotin technique |
摘要 |
A method for preparing an antigen specific probe is provided by incubating a primary antigen specific monoclonal antibody with a biotinylated secondary antibody to produce a complex of the primary and secondary antibodies. The staining pattern produced by these probes reflects the specificity of the monoclonal antibody in the complex and the labeling of irrelevant, endogenous immunoglobulins is reduced substantially. This novel, indirect immunohistochemical method can be used to study normal and diseased tissues using a variety of monoclonal antibodies.
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申请公布号 |
US5281521(A) |
申请公布日期 |
1994.01.25 |
申请号 |
US19920915919 |
申请日期 |
1992.07.20 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
TROJANOWSKI, JOHN Q.;LEE, VIRGINIA M-Y. |
分类号 |
G01N33/531;G01N33/535;(IPC1-7):G01N33/532;G01N33/577 |
主分类号 |
G01N33/531 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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