发明名称 |
THIOBENZOYLATION METHOD OF PEPTIDE SEQUENCING WITH GAS CHROMATOGRAPHY AND MASS SPECTROMETRIC DETECTION |
摘要 |
A novel protein microsequencing method for use in conjunction with thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4(H)) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy. |
申请公布号 |
WO9402844(A1) |
申请公布日期 |
1994.02.03 |
申请号 |
WO1993US05992 |
申请日期 |
1993.06.17 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
STOLOWITZ, MARK, L. |
分类号 |
C07K1/12;G01N30/02;G01N33/68;(IPC1-7):G01N30/72 |
主分类号 |
C07K1/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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