发明名称 |
Method for determination of components |
摘要 |
The present invention relates to a method for quantitative determination of NAD(P)H derived from a specific component, which comprises converting NAD(P)H present in a sample or formed from components other than the objective component by reactions into NAD(P) by the action of glutathione of oxidation type and glutathione reductase; decomposing the remaining glutathione of oxidation type by the action of (gamma)-glutamyl transpeptidase in the presence or in the absence of a mercapto compound; forming NAD(P)H from the component to be determined in the sample utilizing an NAD(P)H-forming reaction system; and quantitatively determining NAD(P)H. According to the method of the present invention, amylase activity, etc. in vital components containing maltose and glucose can be determined accurately.
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申请公布号 |
US5302513(A) |
申请公布日期 |
1994.04.12 |
申请号 |
US19910727369 |
申请日期 |
1991.07.09 |
申请人 |
KYOWA MEDEX CO., LTD. |
发明人 |
MIIKE, AKIRA;TATANO, TOSHIO |
分类号 |
C12Q1/00;C12Q1/40;C12Q1/48;(IPC1-7):C12Q1/48;C12Q1/26;C12Q1/37;C12Q1/52 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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