| 摘要 |
A capillary electrophoresis detector assembly including a separation column in which the components of a mixed sample are separated; an exit column which completes the connection to the terminal buffer reservoir; and a support sleeve which includes precisely positioned radial bores therethrough to allow insertion of optical fibers in the form of an input fiber which transmits the incoming radiation from an illumination source and an exit fiber which receives the radiation and relays same to a detector. In fabricating the assembly, a precision wire is partially inserted into the output end of the separation column. The column/wire combination is inserted into one end of the sleeve. The exit column is threaded onto the precision wire and also inserted into the opposite end of the sleeve. The input and exit fibers are inserted into the radial bores in the sleeve until they touch the precision wire in opposition. Thus, the precision wire serves to establish the spacing between the optical fibers (as determined by the diameter of the wire). This technique affords a known, reproducible, optical path distance between the ends of the fibers. The capillary columns are further slide into the sleeve until they touch the optical fibers. The assembly of the sleeve and fibers is then bonded together whereupon the precision wire is removed.
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