| 摘要 |
The invention provides a clinical-grade placenta mesenchymal stem cell preparation method. Specifically, the method includes: using a combined digestive enzyme to digest a placenta tissue material after being cut up to obtain a digested tissue mixture, wherein the combined digestive enzyme comprises collagenase IV and trypsase; adding a red blood cell lysis buffer to obtain a mixture after going through red blood cell lysis treatment; centrifuging to obtain precipitate, namely primary-generation mesenchymal stem cells; performing serum-free passage amplification on the primary-generation mesenchymal stem cells obtained in the previous step to obtain clinical-grade placenta mesenchymal stem cells after going through passage. The cells prepared by the method are high in purity and proliferation capability and can guarantee needs on clinical use of the cells. |
