| 摘要 |
A method of synthesis of taxanes from pseudocallus of Taxus species, particularly T. baccata , and methods for pseudocallus production are described. Pseudocallus of the invention can be distinguished from callus tissue and cell suspensions by (1) its unique morphology which is characterized by an amorphous aggregation of cells lacking both differentiated tissues and clearly defined meristematic zones; by (2) very poor intercellular adhesion resulting in marked tissue friability; and by (3) relatively high initial rate of mass doubling. Production of taxanes is achieved by culturing the pseudocallus on support culture medium.
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