Nucleic acid tagged immunoassay

摘要

Provided is a method for detecting the presence of an antigen in a specimen from a subject comprising the steps of (a) binding antigens in a specimen from the subject to a solid support; (b) contacting the bound antigens with a specifically immunoreactive ligand, wherein the ligand is linked to an oligonucleotide, having a known sequence, under conditions that permit reaction of the oligonucleotide-linked ligand with the bound antigen; (c) removing unreacted oligonucleotide-linked ligand; and (d) detecting the presence of the oligonucleotide, the presence of the oligonucleotide indicating the presence of the antigen in the subject. A method for detecting the presence of an antibody in a specimen from a subject is also provided. A composition comprising an immunoreactive polypeptide linked to an oligonucleotide is also provided.