IN SITU NUCLEIC ACID AMPLIFICATION

摘要

A method of amplification of nucleic acid which comprises treating a liquid suspension of cells or other cell-like structures containing nucleic acid within a natural or artificial membrane or coat protein, under conditions which maintain cell morphology, with an amplification reaction mixture designed to amplify a target nucleic acid sequence therein and continuing the amplification reaction for at least two rounds of amplification of said target sequence in situ.