发明名称 |
PROCESS FOR CELL ABLATION USING TRANS-SPLICING RIBOZYMES |
摘要 |
PCT No. PCT/US92/00277 Sec. 371 Date Jul. 14, 1993 Sec. 102(e) Date Dec. 23, 1993 PCT Filed Jan. 16, 1992 PCT Pub. No. WO92/13089 PCT Pub. Date Aug. 6, 1992The design of new ribozymes capable of self-catalyzed trans-splicing which are base a upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described. |
申请公布号 |
HUT72183(A) |
申请公布日期 |
1996.03.28 |
申请号 |
HU19930002049 |
申请日期 |
1992.01.16 |
申请人 |
GENERAL HOSPITAL CORP.;PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
HASELOFF,JAMES;GOODMAN,HOWARD M.;BRAND,ANDREA;PERRIMON,NORBERT |
分类号 |
C12N1/21;A61K38/00;C07K7/06;C12N;C12N5/10;C12N7/00;C12N9/10;C12N15/09;C12N15/113;C12N15/52;C12N15/66;C12N15/82;C12N15/85;C12N15/90;(IPC1-7):C12P19/34;C07H15/12;C12N15/00;C12N7/01 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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