A fusion protein is prepared containing a polypeptide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with an affinity matrix containing a substrate such as cellulose for the cellulase substrate binding region, the substrate binding region binds to the affinity matrix to immobilize the polypeptide. The polypeptide can be purified by separating the fusion protein or polypeptide from the affinity matrix.
申请公布号
AT142699(T)
申请公布日期
1996.09.15
申请号
AT19890907798T
申请日期
1989.06.28
申请人
THE UNIVERSITY OF BRITISH COLUMBIA;KILBURN, DOUGLAS G.;MILLER, ROBERT C.;WARREN, RICHARD A.J.;GILKES, NEIL R.
发明人
KILBURN, DOUGLAS, G.;MILLER, ROBERT, C.;WARREN, RICHARD, A., J.;GILKES, NEIL, R.