发明名称 |
Non-infectious, replication defective, immunogenic HIV retrovirus-like particles produced from a recombinant HIV genome devoid of long terminal repeats |
摘要 |
This invention is directed towards nucleic acid molecules capable of producing human immunodeficiency virus (HIV) retrovirus-like particles, which are non-infectious, replication defective, and immunogenic. Recombinant HIV genomes were generated that are devoid of long terminal repeats (LTRs) but contain a heterologous, inducible metallothionein promoter. Additional modifications have been made to the primer binding site, pol, vif, and env coding regions. Upon transfection into a suitable host these DNA molecules are capable of producing HIV retrovirus-like particles that lack genomic RNA. These non-infectious particles will provide suitable antigens for HIV diagnostic assays and immunogenic preparations.
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申请公布号 |
US5571712(A) |
申请公布日期 |
1996.11.05 |
申请号 |
US19940239427 |
申请日期 |
1994.05.06 |
申请人 |
CONNAUGHT LABORATORIES LIMITED |
发明人 |
HAYNES, JOEL;KLEIN, MICHEL H.;ROVINSKI, BENJAMIN;CAO, SHI X. |
分类号 |
C12N7/00;A61K39/00;A61K39/21;A61P31/12;C07K14/155;C07K14/16;C12N15/09;C12N15/48;C12N15/49;C12P21/00;C12P21/02;C12Q1/70;C12R1/91;G01N33/569;(IPC1-7):C12P21/06;C07H21/04 |
主分类号 |
C12N7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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