发明名称 |
OPTIMIZED GENE EDITING UTILIZING A RECOMBINANT ENDONUCLEASE SYSTEM |
摘要 |
Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems. |
申请公布号 |
WO2016183448(A1) |
申请公布日期 |
2016.11.17 |
申请号 |
WO2016US32367 |
申请日期 |
2016.05.13 |
申请人 |
UNIVERSITY OF SOUTHERN CALIFORNIA |
发明人 |
JUNGE, Jason;HUNT, Timothy;FRASER, Scott |
分类号 |
C12N9/22;C12N15/10;C12N15/85 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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