| 摘要 |
<p>An anchor library is described. A collection of recombinant vectors having a nucleic acid encoding a displayed peptide sequence is provided. The displayed peptide sequence of each of the vectors comprises X?1(Y1)c1X2(Y2)c2X3(Y3)c3X4¿, wherein each X?1, X2, X3 and X4¿ is an amino acid residue and any of X?1, X2, X3 and X4¿ can be the same or different from any one other, wherein each Y?1, Y2 and Y3¿ is alanine or glycine or a combination of alanine and glycine that is respectively c?1, c2 and c3¿ amino acid residues long and any of Y?1, Y2 and Y3¿ if present can be the same or different from any one other, wherein each of c?1, c2 and c3¿ is 0 to about 20, wherein X?1 and X4¿ are each attached to an amino acid residue that flanks the displayed peptide sequence. Preferably, at least about 105 to about 108 permutations of all possible permutations of the displayed peptide sequence are present in the anchor library. Preferably, the library does not contain more than about 10 % of displayed peptide sequences different from the first mentioned displayed peptide sequences. Also described are methods of making anchor libraries and methods of using anchor libraries to identify a peptide sequence that binds to a target. Recombinant vectors, filamentous phage, nucleic acid molecules and proteins are also provided.</p> |
