Method of producing human monoclonal antibodies and their use
摘要
Production of human monoclonal antibodies comprises: (a) immortalising human antibody-producing B cells with a modified Epstein-Barr virus (EBV); (b) screening the immortalised B cells for specificity to a target antigen; (c) isolating clones that produce the corresponding antibody, Äculturing the clonesÜ, and (d) recovering the antibodies from the culture supernatants. The modified EBV has a nuclear antigen 2 (EBNA2) gene that can be switched on for the immortalisation step and switched off before the antibody recovery step.
申请公布号
EP0773228(A1)
申请公布日期
1997.05.14
申请号
EP19960117361
申请日期
1996.10.29
申请人
GSF-FORSCHUNGSZENTRUM FUER UMWELT UND GESUNDHEIT, GMBH
发明人
BORNKAMM, GEORG WILHELM, PROF.;EICK, DIRK, PRIV. DOZ. DR.;KEMPKES, BETTINA, DR.;JOCHNER, NICOLA MARIA