CHAIN REACTION CLONING

摘要

Chain reaction cloning methods and reagents and kits for performing such methods are disclosed. Chain reaction cloning methods provide a convenient one step process that will allow site-specific ligation of DNA molecules by thermostable DNA ligases. A number of applications are disclosed for chain reaction cloning including site-specific ligation of DNA fragments generated by restriction enzyme digestion, DNAse digestion, chemical cleavage, enzymatic or chemical synthesis, and PCR amplification.